Archives par étiquette : Matrisome

Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis

Naba A, Clauser K.R,and Hynes R.O. Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis. J. Vis. Exp, 2015, (101), e53057, doi:10.3791/53057

Lien vers la vidéo: ici

Lien vers le protocole: ici

The Extracellular Matrix: Tools and Insights for the “Omics” Era

Naba A, Clauser K.R, Ding H, Whittaker C.A, Carr S.A, and Hynes R.O. The Extracellular Matrix: Tools and Insights for the « Omics » Era. Matrix Biology, 2015, pii: S0945-053X(15)00121-3

Liens vers l’article (open access): http://www.sciencedirect.com/science/article/pii/S0945053X15001213

Highlights:
• The matrisome is defined as the ensemble of 1000 + genes encoding ECM and ECM-associated proteins.

• Bioinformatic and experimental approaches to study the ECM/matrisome are discussed.

• We introduce a novel website and database MatrisomeDB to centralize resources on the matrisome.

• We present a draft of an ECM atlas compiling proteomics data on the ECM of 14 different tissues and tumors.

• “Omics” data provide novel insights into ECM functions in development, homeostasis and disease.